Trihybrid Cross Calculator - Punnett Square

Generate a trihybrid 3-trait Punnett square (64 cells) with offspring genotype frequencies and probabilities.

A trihybrid cross Punnett square models inheritance of 3 traits using 6 parental alleles, producing a 64-cell table of offspring combinations.

This calculator is used to estimate genotype probability for combinations of three traits by counting occurrences in the Punnett square and dividing by total combinations.

It shows the 8x8 Punnett square, genotype frequencies, and percentages. Genotype probability formula: (number of genotype occurrences / total combinations) × 100.

Trihybrid cross quick facts: 64 boxes in the table, 27 possible genotypes, and up to 729 trihybrid configuration variants.

A, B, C - Dominant alleles
a, b, c - Recessive alleles

Mother's traits

Trait 1

A gene

Trait 2

B gene

Trait 3

C gene

Father's traits

Trait 1

A gene

Trait 2

B gene

Trait 3

C gene

Offspring genotype frequency

AaBbCc

12.50%

aaBbCc

6.25%

AabbCc

6.25%

AaBbcc

6.25%

AaBbCC

6.25%

AaBBCc

6.25%

AABbCc

6.25%

aabbCc

3.13%

aaBbcc

3.13%

aaBbCC

3.13%

aaBBCc

3.13%

Aabbcc

3.13%

AabbCC

3.13%

AaBBcc

3.13%

AaBBCC

3.13%

AAbbCc

3.13%

AABbcc

3.13%

AABbCC

3.13%

AABBCc

3.13%

aabbcc

1.56%

aabbCC

1.56%

aaBBcc

1.56%

aaBBCC

1.56%

AAbbcc

1.56%

AAbbCC

1.56%

AABBcc

1.56%

AABBCC

1.56%

Punnett square

♂\♀	ABC	ABc	AbC	Abc	aBC	aBc	abC	abc
ABC	AABBCC	AABBCc	AABbCC	AABbCc	AaBBCC	AaBBCc	AaBbCC	AaBbCc
ABc	AABBCc	AABBcc	AABbCc	AABbcc	AaBBCc	AaBBcc	AaBbCc	AaBbcc
AbC	AABbCC	AABbCc	AAbbCC	AAbbCc	AaBbCC	AaBbCc	AabbCC	AabbCc
Abc	AABbCc	AABbcc	AAbbCc	AAbbcc	AaBbCc	AaBbcc	AabbCc	Aabbcc
aBC	AaBBCC	AaBBCc	AaBbCC	AaBbCc	aaBBCC	aaBBCc	aaBbCC	aaBbCc
aBc	AaBBCc	AaBBcc	AaBbCc	AaBbcc	aaBBCc	aaBBcc	aaBbCc	aaBbcc
abC	AaBbCC	AaBbCc	AabbCC	AabbCc	aaBbCC	aaBbCc	aabbCC	aabbCc
abc	AaBbCc	AaBbcc	AabbCc	Aabbcc	aaBbCc	aaBbcc	aabbCc	aabbcc