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Biology Calculators

Dihybrid Cross Calculator - Punnett Square

Calculate genotype probabilities for two-trait inheritance using a 4x4 Punnett square and parent trait genotypes.

Use this dihybrid cross calculator to model inheritance for two traits (A/a and B/b) at the same time.

Pick mother and father genotypes for trait 1 and trait 2, then combine gametes in a 4x4 Punnett square to compute offspring genotypes.

Resulting probabilities are shown for the 9 possible genotype groups: AABB, AABb, AAbb, AaBB, AaBb, Aabb, aaBB, aaBb, and aabb.

For each cell, offspring genotype is formed by pairing alleles at each locus with dominant letter first (e.g., A with a gives Aa).

A, B – Dominant alleles
a, b – Recessive alleles

Mother's traits

Trait 1
Trait 2

Father's traits

Trait 1
Trait 2

Resulting probabilities (%)

AABB
%
AABb
%
AAbb
%
AaBB
%
AaBb
%
Aabb
%
aaBB
%
aaBb
%
aabb
%

Punnett square

♂\♀ABAbaBab
ABAABBAABbAaBBAaBb
AbAABbAAbbAaBbAabb
aBAaBBAaBbaaBBaaBb
abAaBbAabbaaBbaabb