Biology Calculators
Dihybrid Cross Calculator - Punnett Square
Calculate genotype probabilities for two-trait inheritance using a 4x4 Punnett square and parent trait genotypes.
Use this dihybrid cross calculator to model inheritance for two traits (A/a and B/b) at the same time.
Pick mother and father genotypes for trait 1 and trait 2, then combine gametes in a 4x4 Punnett square to compute offspring genotypes.
Resulting probabilities are shown for the 9 possible genotype groups: AABB, AABb, AAbb, AaBB, AaBb, Aabb, aaBB, aaBb, and aabb.
For each cell, offspring genotype is formed by pairing alleles at each locus with dominant letter first (e.g., A with a gives Aa).
A, B – Dominant alleles
a, b – Recessive alleles
Mother's traits
Trait 1
Trait 2
Father's traits
Trait 1
Trait 2
Resulting probabilities (%)
AABB
%
AABb
%
AAbb
%
AaBB
%
AaBb
%
Aabb
%
aaBB
%
aaBb
%
aabb
%
Punnett square
| ♂\♀ | AB | Ab | aB | ab |
|---|---|---|---|---|
| AB | AABB | AABb | AaBB | AaBb |
| Ab | AABb | AAbb | AaBb | Aabb |
| aB | AaBB | AaBb | aaBB | aaBb |
| ab | AaBb | Aabb | aaBb | aabb |